c-Myc Mouse mAb
- 貨號 Cat. No.
- OP30
- 規格 Size
- 100 µl
Description
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| Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions. Full details are available at www.calbiochem.com. |
| Siz | 100 μg |
| Applications: | Immunoblotting (1-5 μg/ml) |
| Description: | Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2 mouse myeloma cells. Recognizes the ~64-67 kDa (apparent MW) c-Myc protein. |
| Background: | The v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc and B-myc. In both mouse plasmacytomas and human Burkitt's lymphoma, coding sequences from the c-myc gene are translocated from their normal chromosomal position into a transcriptionally active immunoglobulin locus on another chromosome. Amplification of the c-myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-myc gene has been found amplified in neuroblastomas, small cell lung carcinomas and Wilms tumors. L-myc is amplified in small cell lung cancer. Furthermore, elevated levels of myc mRNA and protein have been demonstrated in human tumors even in the absence of gene amplification. Immunological studies have shown that the human c-myc gene gives rise to at least two nuclear phosphoproteins of ~64 kDa and ~67 kDa that exhibit relatively short (30 min) half lives in vivo and exhibit DNA binding properties in vitro. |
| Host: | Mouse |
| Immunogen: | Recombinant, human c-Myc |
| Clone: | 8 |
| Isotype: | IgG2a |
| Known Species Reactivity: | Avian, human, mouse |
| Positive Control: | HL-60 cells |
| Negative Control: | HT1080 cells |
| Form: | Liquid |
| Formulation: | In 0.05 M sodium phosphate buffer, 0.2% gelatin. Please see label for lot-specific concentration. |
| Preservative: | ≤0.1% sodium azide |
| Storage: | 2 to 8°C. Do not freeze. |
| Toxicity: | MSDS available upon request. |
| Comments: | The c-Myc protein is extremely labile. c-Myc can be degraded during a freeze/thaw cycle; thus, we recommend using fresh lysates with a cocktail of protease inhibitors. c-Myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. The sequence of c-Myc predicts a ~45 kDa protein, but c-Myc migrates under reducing conditions as a ~64-67 kDa band. This is the preferred antibody for immunoblotting. For best results with immunoprecipitation, compare results with and without SDS. Antibody should be titrated for optimal results in individual systems. |
| References: | LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144. |