Bovine Factor V/Va

Purified from freshly collected bovine plasma using a combination of salt precipitations and column chromatography. This protein is a mixture of Factor V/Va and intermediate activation products as judged by 4-20% gradient gels. Bovine Factor Va functions as a cofactor with the serine protease Factor Xa in the activation of prothrombin. This protein may be used as a source of V/Va in the Prothrombinase complex.

Buffer composition = 20 mM HEPES-HCl/0.15 M NaCl/pH 7.4/50% glycerol

*Extinction Coefficient (1%) = 9.6/17.4

One unit = 10 µg

Molecular Weight = 330,000; 94,000; 74,000; 71,000; 31,000 daltons

Concentration determined by BCA