Bovine A1 Beta Casein ELISA Reagent Set (10 plates)

ZZZZ_Status (Active, Disc) : Active

 

AA_Supplier Name : Biosensis

 

Alternative_Name (Other Names) :

Beta Casein; CSN2

 

Antibody_Isotype
(Antibody only) :

 

Application_Details :

Please download the detailed product insert for complete instructions for the successful use of this ELISA; Use only as directed.

 

Background_Info : The protein named Casein is the largest group of proteins in mammalian milk, making up about > 80% of total protein content. Different mammals have different amounts of caseins in their milks and not all casein proteins are the same.

According to published literature, (PMID: 15453478), multiple forms of casein proteins exist in cow's milk, with beta-casein being one of the most predominant forms. Beta casein in domestic cows at lesat, has two alleles or isoforms. Beta A1 and Beta A2.

A1 beta-casein is found in milk from breeds of cows that originated in northern Europe. These breeds include Holstein, Friesian, Ayrshire, and British Shorthorn.
A2 beta-casein. is found in milk breeds that originated in the Channel Islands US and southern France. These include Guernsey, Jersey, Charolais, and Limousin cows (PMID: 15867940, PMID: 15453478). .
Traditionally, store bought regular milk contains both A1 and A2 beta-casein proteins, but pure A2 milk can be obtained that originates only from A2 cows.

Some studies suggest that there could be some sort of a link between the A1 beta-casein and immunological dieseases such as type-1 diabetes and others and work is underway to understand how beta casein, both A1 and A2 may or may not contribute to human illness as well a health.

 

Clone_Name
(AB only, monoclonals) :

 

Datasheet_Link : http://www.biosensis.com/pdf_datasheet.php?products_id=1723

 

Description (Long Description) : 

Bovine milk may contain two types of beta-casein protein, A2 or A1, which differ by a single-point mutation at amino acid position 82 (P82/H82). Recent studies have shown that milk containing the A1 beta casein protein can contribute to issues including gastrointestinal discomfort after ingestion. There is some evidence of a link between ingestion of A1 beta casein protein and the development of Type 1 diabetes. The Biosensis Bovine A1 Beta Casein ELISA Kit is a sandwich ELISA that allows the specific quantification of the A1 isoform of bovine beta casein in bovine milk, only if used as directed. The A2 isoform is not detected in this ELISA assay. Please refer to the kit protocol for specific use instructions. A matched pair of antibodies for capture and specific detection of A1 beta casein, native A1 protein standard and HRP-labelled secondary antibody is provided to coat and run 10 x 96-well microplates. Precise instructions are given to ensure consistent assay performance.

 

Expiry_Date : 

If the reagents will not be used with 1 week of receipt, then the -20°C components should be stored in -10°C to -20°C freezer. Expiration of the unopened set of reagents is 12 months if stored as directed in insert that comes with the kit. Once opened, use all components within 1 week. Place components in appropriate temperature locations as directed below. For -20°C items multiple freeze-thaw cycles are NOT recommended. Divide into aliquots if not using all thawed material immediately

 

Format (Form) : 

 

Immunogen (Antigen) :Standard is purified bovine A1

 

Kit_Components : Rabbit anti-bovine beta casein polyclonal capture antibody, 1 mg/mL, 500 ug (1 tube);Chicken anti-bovine A1 beta casein polyclonal detection antibody, 0.5 mg/mL, 10 uL/tube (10 tubes);Native bovine A1 beta-casein standard, 40 ug/mL, 20 uL/tube (10 tubes);Rabbit anti-chicken IgY HRP-conjugate, 1000X, 20 uL/tube (10 tubes)
Reagents required, but not included in this kit: 96-well microplates;Coating buffer;TBS and TBST buffer;Tween-20;BSA;TMB substrate;1M HCl;0.5M NaOH

 

MSDS (File name) :