bostonbiochem.com/product/b-110
- 貨號 Cat. No.
- B-110
- 規格 Size
- 0.5 ml
- 品牌 Brand
- Boston Biochem
Description
200X Deconjugation Stop Buffer
Product Information
0.5 ml
0.5 ml of 100mM NEM (N-Ethylmaleimide)
Use & Storage
Store at -20°C. Avoid multiple freeze/thaw cycles.
Catalog #: B-110
200X Deconjugation Stop Buffer
This buffer contains the optimal formulation for the general inhibition of enzyme activities that deconjugate ubiquitin and ubiquitin-like (UBL) protein (SUMO, NEDD8, ISG15) from protein substrates. Useful during the preparation of crude lysate samples to enrich for protein conjugates or to quench in vitro assays. This agent will also quench the activities of other non-related cycsteine proteases. More specific deconjugating enzyme inhibitor include aldehyde and vinyl sulfone derivatives such as ubiquitin (U-201, U-202), SUMO (UL-701, UL-702), NEDD8 (UL- 801, UL-802) and ISG15 (UL-602, UL-603).
Product Information
Quantity:
0.5 ml
Stock:
0.5 ml of 100mM NEM (N-Ethylmaleimide)
Use & Storage
Use:
Dilute to 1X in reaction to stop conjugation activity for final concentration of 0.5μM in assay.
Storage:
Store at -20°C. Avoid multiple freeze/thaw cycles.