Boc-Leu-Arg-Arg-AMC (Boc-LRR-AMC)
- 貨號 Cat. No.
- S-300
- 規格 Size
- 5 mg
- 品牌 Brand
- Boston Biochem
Description
Boc-Leu-Arg-Arg-AMC (Boc-LRR-AMC)
Synthetic
Product Information
5 mg
> 99% as adjudged by thin layer chromatography.
Soluble in methanol up to 50 mg/ml. For best results, pellet dry compound prior to reconstitution. Solubilize at desired stock concentration.
Use & Storage
Add from DMSO stock directly to in vitro or in vivo assay at desired concentration. Typical concentrations range from 10-100 μM. (λex: 380nm; λem: 460 nm)
Store lyophilized powder at -20°C for at least one year. Stock solution at -20°C for up to 1 month. Avoid multiple freeze/thaw cycles.
Catalog #: S-300
Boc-Leu-Arg-Arg-AMC (Boc-LRR-AMC)
Product Source:
Synthetic
Fluorogenic tri-peptide substrate for measuring the "trypsin-like" peptidase activity of the 20S proteasome. Each of the two 20S internal β-type rings harbors three different proteolytically active sites: β1 ("post-glutamyl peptide hydrolase" site), β2 ("trypsin-like" site) and β5 ("chymotrypsin-like" site). This peptide is also substrate for the Kex2 endopeptidase from S. Cervisiae, which has substrate specificity toward the C-terminal side of LR, PR and RR sequences. In addition, the peptide can be cleaved by Kalikrein 5 with a P1 site preference for basic residues (R and K).
Product Information
Quantity:
5 mg
Structure:
Purity:
> 99% as adjudged by thin layer chromatography.
Solubility:
Soluble in methanol up to 50 mg/ml. For best results, pellet dry compound prior to reconstitution. Solubilize at desired stock concentration.
Use & Storage
Use:
Add from DMSO stock directly to in vitro or in vivo assay at desired concentration. Typical concentrations range from 10-100 μM. (λex: 380nm; λem: 460 nm)
Storage:
Store lyophilized powder at -20°C for at least one year. Stock solution at -20°C for up to 1 month. Avoid multiple freeze/thaw cycles.