BIOXYTECH® LPO-586

Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides are unstable, and decompose to form a complex series of compounds including reactive carbonyl compounds. Polyunsaturated fatty acid peroxides generate malonaldehyde (MDA) and 4-hydroxyalkenals upon decomposition. Measurement of malonaldehyde and 4-hydroxyalkenals has been used as an indicator of lipid peroxidation (ESTERBAUER, 1991). The LPO-586 method is designed to assay either MDA alone (in hydrochloric acid ) or MDA in combination with 4-hydroxyalkenals (in methanesulfonic acid).