Biotinylated SARS-CoV-2 (COVID-19) S1 protein, His,Avitag™ (MALS verified)

Source : 

Biotinylated SARS-CoV-2 S1 protein, His,Avitag (S1N-C82E8) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1).

 

Molecule : S1 protein

 

Synonyms : S1 protein,Spike glycoprotein Subunit1,S glycoprotein Subunit1,Spike protein S1,COVID-19

 

Format : Powder

 

Category : MABSol® Biotin Labeled Proteins

 

Accession : QHD43416.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 78.6 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 78.6 kDa. The protein migrates as 100-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

 

References :

(1) Wan Y, et al. J Virol. 2020. pii: JVI.00127-20.
(2) Benvenuto D, et al. J Med Virol. 2020.
(3) Chang CY, et al. AMB Express. 2020. 10(1):20.