Biotinylated Mouse IgG2a Fc Protein, Avitag™

Source : 

Biotinylated Mouse IgG2a Fc, Avitag (IGA-M8210) is expressed from human 293 cells (HEK293). It contains AA Glu 98 - Lys 330 (Accession # P01863).

 

Molecule : IgG2a Fc

 

Synonyms : IgG2A

 

Format : Powder

 

Category : MABSol® Biotin Labeled Proteins

 

Accession : N/A

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 28.2 kDa

 

Characteristics :

This protein carries an Avi tag at the C-terminus. The protein has a calculated MW of 28.2 kDa. The protein migrates as 36 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : Tris with Glycine, Arginine and NaCl, pH7.5

 

Description :

Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis.

 

References :

(1) Sikorav J.-L., et al., 1980, Nucleic Acids Res. 8:3143-3155.
(2) Ollo R., et al., 1981, Proc. Natl. Acad. Sci. U.S.A. 78:2442-2446.