Biotinylated Mouse CD28 Protein, His,Avitag™

Source : 

Biotinylated Mouse CD28, His,Avitag (CD8-M82E3) is expressed from human 293 cells (HEK293). It contains AA Asn 20 - Lys 149 (Accession # P31041-1).

 

Molecule : CD28

 

Synonyms : CD28,Tp44

 

Format : Powder

 

Category : MABSol® Biotin Labeled Proteins

 

Accession : NP_031668.3

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 18.6 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 18.6 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

CD28 homolog (CD28H), also called transmembrane and immunoglobulin domain containing 2 (TMIGD2) and IGPR-1,is encoded by the TMIGD2 gene. CD28H is constitutively expressed on naive T and NK cells. After interaction of CD28 homolog with B7 homologue,the peripheral effector and memory T cells can be activated and proliferated by Akt-dependent signalling cascade. Plays a role in cell-cell interaction, cell migration, and angiogenesis. Through interaction with HHLA2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.

 

References :

(1)Evans E.J., et al., 2005, Nat. Immunol. 6:271-279.
(2) Mayya V., et al., 2009, Sci. Signal. 2:RA46-RA46.
(3) Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-386.