Biotinylated Human TSLP (R127A, R130A) Protein, His,Avitag™

Source : 

Biotinylated Human TSLP (R127A, R130A), His,Avitag (TSP-H82E0) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1 (R127A, R130A)).

 

Molecule : TSLP

 

Synonyms : TSLP

 

Format : Powder

 

Category : MABSol® Biotin Labeled Proteins

 

Accession : NP_149024.1 (R127A, R130A)

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 18.5 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 18.5 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine involved in the pathology of inflammatory skin diseases, and is widely expressed by epithelial cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Human TSLP has been shown to developing nondeletional central tolerance, amplifying epithelium-induced class switching, inducing atopic diseases and maintaining intestinal noninflammatory environment. Among diverse cells responding to Human TSLP, CD11c+ dendritic cells are the most obviously characterized target cells.

 

References :

(1) Segawa R., et al., 2017, J. Dermatol. Sci. S0923-1811:31012-31015.
(2) Wang J., et al., 2008, Cell. Mol. Immunol. 5:99-106.
(3) Soumelis V., et al., 2002, Nat. Immunol. 3:673-680.