Biotinylated Human SCF / KITLG Protein, Avitag™,His Tag

Source : 

Biotinylated Human SCF, Avitag,His Tag (SCF-H82E1) is expressed from human 293 cells (HEK293). It contains AA Glu 26 - Ala 190 (Accession # AAH69797).

 

Molecule : SCF

 

Synonyms : KITLG,FPH2,KL-1,Kitl,MGF,SCF,SF,SHEP7,KL

 

Format : Powder

 

Category : MABSol® Biotin Labeled Proteins

 

Accession : AAH69797

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 21.7 kDa

 

Characteristics :

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 21.7 kDa. The protein migrates as 26-38 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Kit ligand (KITLG) is also known as stem cell factor (SCF), mast cell growth factor (MGF), steel factor (SF), which belongs to the SCF family, and is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. KITLG is the ligand for the receptor-type protein-tyrosine kinase KIT. SCF / MGF plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG / SCF binding can activate several signaling pathways. KITLG / SF Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG / SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG / SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG / SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1, 4, 5 - trisphosphate. KITLG / SCF acts synergistically with other cytokines, probably interleukins.

 

References :

(1) Lu H.S., et al., 1992, Arch. Biochem. Biophys. 298:150-158.
(2) Wang Z.-Q., et al., 2009, Am. J. Hum. Genet. 84:672-677.
(3) Lev S., et al., 1992, J. Biol. Chem. (UNITED STATES) 267 (15): 10866–73.