Biotinylated Human OX40 Ligand / TNFSF4 Protein, Avitag™,Fc Tag

Source : 

Biotinylated Human OX40 Ligand, Avitag,Fc Tag (OXL-H82F4) is expressed from human 293 cells (HEK293). It contains AA Gln 51 - Leu 183 (Accession # P23510-1).

 

Molecule : OX40 Ligand

 

Synonyms : OX40L,TNFSF4,CD252,Glycoprotein Gp34,TXGP1,CD134 ligand,CD134L

 

Format : Powder

 

Category : MABSol® Biotin Labeled Proteins

 

Accession : NP_003317.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 48.7 kDa

 

Characteristics :

This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag. The protein has a calculated MW of 48.7 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : Tris with Glycine, Arginine and NaCl, pH7.5

 

Description :

Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L), TAX transcriptionally-activated glycoprotein 1 and CD252, which belongs to the tumor necrosis factor family. TNFSF4 is the ligand for CD134 and is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation. The interaction of TNFSF4-TNFSF4 is involved in the pathogenesis of multiple autoimmune and inflammatory diseases such as systemic lupus erythematosus (SLE), carotid artery disease and cancer. Furthermore, similar to other TNF superfamily members, membrane-bound OX40 Ligand (TNFSF4) exists as a homotrimer. Human TNFSF4 shares 46% amino acid sequence identity with its mouse counterpart.

 

References :

(1) Compaan D.M., et al., 2006, Structure 14:1321-1330.
(2) Maizels RM., et al., 2003, Nat. Rev. Immunol. 3 (9): 733–44.