Biotinylated Human Neuropilin-1 / NRP1 / CD304 Protein, His,Avitag™

Source : 

Biotinylated Human Neuropilin-1, His,Avitag (NR1-H82E3) is expressed from human 293 cells (HEK293). It contains AA Phe 22 - Lys 644 (Accession # O14786-1 (V179A)).

 

Molecule : Neuropilin-1

 

Synonyms : NRP1,Neuropilin-1,NRP,VEGF165R,CD304

 

Format : Powder

 

Category : MABSol® Biotin Labeled Proteins

 

Accession : NP_001019799.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 73.5 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 73.5 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Neuropilin-1 (NRP1) is also known as Vascular endothelial cell growth factor 165 receptor (VEGF165R), CD antigen CD304, which belongs to the neuropilin family. The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells.

 

References :

(1) Tamagnone L., et al., 1999, Cell 99:71-80.
(2) Appleton B.A., et al., 2007, EMBO J. 26:4902-4912.