Biotinylated Human MIF Protein, His,Avitag™

Source : 

Biotinylated Human MIF, His,Avitag (MIF-H82E7) is expressed from human 293 cells (HEK293). It contains AA Pro 2 - Ala 115 (Accession # P14174-1).

 

Molecule : MIF

 

Synonyms : MIF,GLIF,MMIF,GIF

 

Format : Powder

 

Category : MABSol® Biotin Labeled Proteins

 

Accession : NP_002406.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 16.0 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 16.0 kDa. The protein migrates as 20 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 25 mM MES, 500 mM NaCl, pH6.5

 

Description :

Macrophage migration inhibitory factor(MIF) is also known as Glycosylation-inhibiting factor(GIF), L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase. Interacts with CXCR2 extracellular domain, CD74 extracellular domain, COPS5 and BNIPL. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

 

References :

(1) Fan C, et al. 2013. Proc Natl Acad Sci U S A. 110(27):10994-9.
(2) Emonts M, et al. 2007. Clin Infect Dis. 44(10):1321-8.
(3) Oddo M, et al. 2005. Infect Immun. 73(6):3783-6.