Biotinylated Human LIF Protein, His,Avitag™

Source : 

Biotinylated Human LIF, His,Avitag (LIF-H82E2) is expressed from human 293 cells (HEK293). It contains AA Ser 23 - Phe 202 (Accession # P15018-1).

 

Molecule : LIF

 

Synonyms : LIF,CDF,DIA,HILDA,MLPLI

 

Format : Powder

 

Category : MABSol® Biotin Labeled Proteins

 

Accession : NP_002300.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 23.4 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 23.4 kDa. The protein migrates as 37-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo.
Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells.

 

References :

(1) Wenwei Hu, Zhaohui Feng, Angelika K. Teresky1, Arnold J. Levine (November 29, 2007).
(2) Kawahara Y, Manabe T, Matsumoto M, Kajiume T, Matsumoto M, Yuge L (2009). PLoS ONE 4 (7): e6343.
(3) Patterson PH (1994). Proc. Natl. Acad. Sci. U.S.A. 91 (17).