Biotinylated Human IL-21 Protein, Fc,Avitag™

Source : 

Biotinylated Human IL-21, Fc,Avitag (IL1-H82F7) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Ser 162 (Accession # Q9HBE4-1).

 

Molecule : IL-21

 

Synonyms : IL21,Za11,Interleukin-21

 

Format : Powder

 

Category : MABSol® Biotin Labeled Proteins

 

Accession : NP_001193935.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 43.9 kDa

 

Characteristics :

This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 43.9 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : Tris with Glycine, Arginine and NaCl, pH7.5

 

Description :

Interleukin-21 (IL-21) is a secreted protein which belongs to the IL-15 / IL-21 family. Interleukin-21 / IL-21 belongs to a family of cytokines that bind to a composite receptor consisting of a private receptor (IL21R) and the common cytokine receptor gamma chain (gamma(C)). Interleukin-21 / IL-21 impacts a number of cell types, including CD8+ memory T cells, NK cells and subsets of CD4 memory T cells. The IL-21R is widely distributed on lympho-haematopoietic cells. IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the γ chain (γc) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.

 

References :

(1) Chtanova T, et al., 2004, J Immunol 173 (1): 68–78.
(2) Wei L, et al., 2007, J Biol Chem 282 (48): 34605–10.
(3) Wurster AL, et al., 2002, J Exp Med 196 (7): 969–77.
(4) Iannello A, et al., 2010, J Leukoc Biol 87 (5): 857–67.
(5) Williams L.D., et al., 2010, Journal of Virology 85 (5): 2316–2324.