Biotinylated Human IL-2 Protein, Fc,Avitag™

Source : 

Biotinylated Human IL-2, Fc,Avitag (IL2-H82F3) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153 (Accession # P60568-1).

 

Molecule : IL-2

 

Synonyms : IL2,TCGF,lymphokine,Interleukin 2

 

Format : Powder

 

Category : MABSol® Biotin Labeled Proteins

 

Accession : NP_000577.2

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 43.7 kDa

 

Characteristics :

This protein carries a human IgG1 Fc tag at the N-terminus and an Avi tag at the C-terminus. The protein has a calculated MW of 43.7 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : Tris with Glycine, Arginine and NaCl, pH7.5

 

Description :

Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit crossspecies activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self antigens", which could result in "autoimmunity". T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.

 

References :

(1) Gaffen, S.L. and K.D. Liu, 2004, Cytokine 28:109.
(2) Kasakura S, Lowenstein L, 1965, Nature 208 (5012): 794-795.
(3) Smith KA, Favata MF, Oroszlan S, 1983, J. Immunol. 131 (4): 1808.
(4) Thornton AM, Shevach EM, 1998, J. Exp. Med. 188 (2): 287-96.