Biotinylated Human CTLA-4 Protein, Fc,Avitag™

Source : 

Biotinylated Human CTLA-4, Fc,Avitag (CT4-H82F3) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162 (Accession # P16410-1).

 

Molecule : CTLA-4

 

Synonyms : CTLA4,CD152

 

Format : Powder

 

Category : MABSol® Biotin Labeled Proteins

 

Accession : NP_005205.2

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 41.8 kDa

 

Characteristics :

This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 41.8 kDa. The protein migrates as 48-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : Tris with Glycine, Arginine and NaCl, pH7.5

 

Description :

CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.

 

References :

(1) Waterhouse P, et al., 1995, Science 270 (5238): 985–8.
(2) Magistrelli G, et al., 1999. Eur. J. Immunol. 29 (11): 3596–602.
(3) Rudd, CE. et al., 2009, Immunol. Rev. 229 (1): 12-26.