Biotinylated Human CD73 / NT5E Protein, His,Avitag™

Source : 

Biotinylated Human CD73, His,Avitag (CD3-H82E3) is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549 (Accession # P21589-1).

 

Molecule : CD73

 

Synonyms : CD73,NT5E,5'-Nucleotidase,5'-NT,NT5,NTE

 

Format : Powder

 

Category : MABSol® Biotin Labeled Proteins

 

Accession : NP_002517.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 61.7 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 61.7 kDa. The protein migrates as 42 kDa and 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : 20 mM Tris, 120 mM NaCl, pH7.5

 

Description :

5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

 

References :

(1)Misumi Y., et al., 1990, Eur. J. Biochem., 191 (3): 563-569.
(2)St. Hilaire, Cynthia, et al., 2011, New England Journal of Medicine, 364 (5): 432-442.
(3)Sharp, J., 1954, Ann. Rheum. Dis., 13 (1): 15-27.