Biotinylated Human CD4 Protein, His,Avitag™

Source : 

Biotinylated Human CD4, His,Avitag (CD4-H82E8) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Pro 396 (Accession # P01730-1).

 

Molecule : CD4

 

Synonyms : CD4,CD4mut,LEU3

 

Format : Powder

 

Category : MABSol® Biotin Labeled Proteins

 

Accession : NP_000607.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 45.0 kDa

 

Characteristics :

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 45.0 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

T-cell surface glycoprotein CD4 is also known as T-cell surface antigen T4/Leu-3. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is accessory protein for MHC class-II antigen/T-cell receptor interaction. CD4 induces the aggregation of lipid rafts. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Therefore, medical professionals refer to the CD4 count to decide when to begin treatment for HIV-infected patients.

 

References :

(1) Brady RL, et al., 1993, Science 260 (5110): 979–83.
(2) Zeitlmann, L., et al., 2001, J. Biol. Chem. (United States) 276 (12): 9123–32.
(3) Rudd CE, et al., 2010, J. Immunol. 185 (5): 2645–9.
(4) Rudd CE, et al., 1988, Proc. Natl. Acad. Sci. U.S.A. 85 (14): 5190–4.
(5) Gorska MM, et al., 2004, J. Exp. Med. 199 (3): 369–79.