Biotinylated Human CA125 / MUC16 Protein, Fc,Avitag™

Source : 

Biotinylated Human CA125, Fc,Avitag (CA5-H82F4) is expressed from human 293 cells (HEK293). It contains AA Gly 12660 - Met 12923 (Accession # Q8WXI7-1).

 

Molecule : CA125

 

Synonyms : CA125,CA-125,CA125MUC-16,FLJ14303,MUC16,mucin 16,mucin-16

 

Format : Powder

 

Category : MABSol® Biotin Labeled Proteins

 

Accession : NP_078966.2

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 56.9 kDa

 

Characteristics :

This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™). The protein has a calculated MW of 56.9 kDa. The protein migrates as 75-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : Tris with Glycine, Arginine and NaCl, pH7.5

 

Description :

The CA125, also known as the MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. The CA 125 molecule is almost certainly a glycoprotein with a predominance of O-linkages. It is heterogeneous with regard to both size and charge, most likely due to continuous deglycosylation of side chains during its life-span in bodily fluids. It exists as a very large complex (perhaps as much as 4 million daltons) under natural conditions.

 

References :

(1) O'Brien TJ., et al. 2002, Tumour Biol., 23(3), 154-69.
(2) O'Brien TJ., et al. 1998, Int J Biol Markers., 13(4), 188-95.
(3) Yin BW., et al. 2001, J Biol Chem., 276(29), 27371-5.