Biotinylated Human B7-H3 / CD276 Protein, Avitag™,His Tag

Source : 

Biotinylated Human B7-H3, Avitag,His Tag (B73-H82E6) is expressed from human 293 cells (HEK293). It contains AA Leu 29 - Pro 245 (Accession # Q5ZPR3-2).

 

Molecule : B7-H3

 

Synonyms : B7-H3,CD276,B7 homolog 3

 

Format : Powder

 

Category : MABSol® Biotin Labeled Proteins

 

Accession : NP_079516.1

 

Storage : -20℃

 

Shipping condition : Powder,RT

 

Molecular Weight : 26.5 kDa

 

Characteristics :

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 26.5 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

 

Endotoxin Level : Less than 1.0 EU per μg by the LAL method.

 

Buffer : PBS, pH7.4

 

Description :

B7 homolog 3 (B7-H3), a member of the immunoglobulin superfamily, is also known CD276, which contains two Ig-like C2-type (immunoglobulin-like) domains and two Ig-like V-type (immunoglobulin-like) domains. B7-H3 may participate in the regulation of T-cell-mediated immune response. B7-H3 also plays a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. Furthermore, B7-H3 is involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. It could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy.

 

References :

(1) Chapoval A.I., et al., 2001, Nat. Immunol. 2:269-274.
(2) Castriconi R., et al., 2004, Proc. Natl. Acad. Sci. U.S.A. 101:12640-12645.
(3) Wang L., et al., 2005, Eur. J. Immunol. 35:428-438.