Biotin-NEDD8
- 貨號 Cat. No.
- UL-820
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
Biotin-NEDD8
human recombinant
Product Information
50 μg
X mg/ml (X μM) in 20 mM HEPES pH 8.0. Actual concentration will vary with specific Lot #.
9 kDa
> 95% by SDS-PAGE
Use & Storage
Store at -80°C. Avoid multiple freeze/thaw cycles.
Catalog #: UL-820
Biotin-NEDD8
Product Source:
human recombinant
NEDD8 modified with biotin via primary amine coupling is ideal as an alternative to radiolabeled NEDD8. This results in multiple biotinylated NEDD8 species with modified lysines as well as the N-terminus. Biotinylated NEDD8 can be detected using avidin-linked reagents. The ubiquitin-like protein NEDD8 is conjugated to targets by the NEDD8-specific E1 activating enzyme (AppBp1/Uba3), the UbcH12 E2 enzyme, and the ROC1/Rbx1 RING FINGER E3 ligase. NEDD8 plays a critical regulatory role in cell proliferation and development, and modifies nearly all members of the cullin family.
Product Information
Quantity:
50 μg
Stock:
X mg/ml (X μM) in 20 mM HEPES pH 8.0. Actual concentration will vary with specific Lot #.
MW:
9 kDa
Purity:
> 95% by SDS-PAGE
Use & Storage
Use:
Suggested concentration for in vitro assays is 5-25 μM.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.