Biotin-N-terminal Ubiquitin, No Lysines
- 貨號 Cat. No.
- UB-NOK
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
Biotin-N-terminal Ubiquitin, No Lysines
human recombinant
Product Information
50 μg
Soluble in aqueous buffers up to 5 mg/ml.
8.6 kDa
> 95% by PAGE
This is a lyophilized powder
Use & Storage
Depending on desired signal strength and assay conditions, biotin-N-term Ub should be used in conjunction with native ubiquitin at combined concentration range of 10-50 μM with a 1:2 to 1:20 ratio of biotin-N-term Ub: native Ub, respectively. Typical amounts for a 20 μL reaction: 250 ng to 2 μg biotin-Nterm- Ub, 5 μg native ubiquitin. Poly-ubiquitin chain visualization/quantitation can be performed via avidin-linked detection.
Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.
Catalog #: UB-NOK
Biotin-N-terminal Ubiquitin, No Lysines
Product Source:
human recombinant
Produced via a proprietary process resulting in a single biotin modification exclusively on the Nterminus of ubiquitin. This site-specific modification results in an ubiquitin that is fully functional at the C-terminus. This ubiquitin mutant lacks all reactive lysine residues which have been mutated to arginine. These mutations render the protein unable to form poly-ubiquitin chains and can be used as a negative control or to detect mono-ubiquitination. Detection with avidin-linked reagents allows for a higher efficiency and detection sensitivity than with antiubiquitin antibodies. Ideal as an alternative to radio-labeled ubiquitin.
Product Information
Quantity:
50 μg
Stock:
Soluble in aqueous buffers up to 5 mg/ml.
MW:
8.6 kDa
Purity:
> 95% by PAGE
Supplied:
This is a lyophilized powder
Use & Storage
Use:
Depending on desired signal strength and assay conditions, biotin-N-term Ub should be used in conjunction with native ubiquitin at combined concentration range of 10-50 μM with a 1:2 to 1:20 ratio of biotin-N-term Ub: native Ub, respectively. Typical amounts for a 20 μL reaction: 250 ng to 2 μg biotin-Nterm- Ub, 5 μg native ubiquitin. Poly-ubiquitin chain visualization/quantitation can be performed via avidin-linked detection.
Storage:
Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.