Biotin-N-terminal Ubiquitin, K48
- 貨號 Cat. No.
- UB-K480
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
Biotin-N-terminal Ubiquitin, K48
human recombinant
Product Information
50 μg
Soluble in aqueous buffers up to 20 mg/ml.
8.6 kDa
> 95% by PAGE
This is a lyophilized powder
Use & Storage
Depending on desired signal strength and assay conditions, biotin-N-term Ub should be used in conjunction with native ubiquitin at combined concentration range of 10-50 μM with a 1:2 to 1:20 ratio of biotin-N-term Ub: native Ub, respectively. Typical amounts for a 20 μL reaction: 250 ng to 2 μg biotin-Nterm- Ub, 5 μg native ubiquitin. Poly-ubiquitin chain visualization/quantitation can be performed via avidin-linked detection.
Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.
Catalog #: UB-K480
Biotin-N-terminal Ubiquitin, K48
Product Source:
human recombinant
Produced via a proprietary process resulting in a single biotin modification exclusively on the N- terminus of ubiquitin. This site-specific modification results in an ubiquitin that is fully functional at the C-terminus, and with the full compliment of reactive lysines to allow for poly- ubiquitin chain incorporation. Detection with avidin-linked reagents allows for a higher efficiency and detection sensitivity than with anti-ubiquitin antibodies. Ideal as an alternative to radio-labeled ubiquitin. This ubiquitin mutant contains only a single lysine (K48) with all other lysines mutated to arginine, and is able to form poly-ubiquitin chains with other ubiquitin molecules via the K48 lysine only.
Product Information
Quantity:
50 μg
Stock:
Soluble in aqueous buffers up to 20 mg/ml.
MW:
8.6 kDa
Purity:
> 95% by PAGE
Supplied:
This is a lyophilized powder
Use & Storage
Use:
Depending on desired signal strength and assay conditions, biotin-N-term Ub should be used in conjunction with native ubiquitin at combined concentration range of 10-50 μM with a 1:2 to 1:20 ratio of biotin-N-term Ub: native Ub, respectively. Typical amounts for a 20 μL reaction: 250 ng to 2 μg biotin-Nterm- Ub, 5 μg native ubiquitin. Poly-ubiquitin chain visualization/quantitation can be performed via avidin-linked detection.
Storage:
Store at -20°C once reconstituted. Avoid multiple freeze/ thaw cycles.