Biotin-Lysozyme Conjugation Substrate
- 貨號 Cat. No.
- SP-100
- 規格 Size
- 50 μg
- 品牌 Brand
- Boston Biochem
Description
Biotin-Lysozyme Conjugation Substrate
hen egg white
Product Information
50 μg
14 kDa
Soluble up to 2 mg/ml in 50 mM Hepes pH 8, 100 mM NaCl.
This is a lyophilized powder
Use & Storage
Store at -80°C once reconstituted. Avoid multiple freeze/ thaw cycles.
> 95% by SDS-PAGE
Catalog #: SP-100
Biotin-Lysozyme Conjugation Substrate
Product Source:
hen egg white
Lysozyme has been used extensively as a substrate to study the ubiquitin-dependent proteolytic pathway in vitro. This protein is an N-end rule substrate, since the recognition of protein requires the binding of an E3 ligase to the N-terminal lysine residue. Ubiquitin-lysozyme conjugates can be formed in the presence of ATP, ubiquitin and the appropriate conjugation enzyme source. This substrate protein can be used as a positive control with crude fractions (F-340, F-360, F-370, F-372) and conjugation kits (K-915, K-930, K-935, K-960). Additionally, this lysozyme is labeled with biotin for the sensitive detection with avidin affinity reagents, but has all available lysines present for the efficient conjugation to ubiquitin.
Product Information
Quantity:
50 μg
MW:
14 kDa
Solubility:
Soluble up to 2 mg/ml in 50 mM Hepes pH 8, 100 mM NaCl.
Supplied:
This is a lyophilized powder
Use & Storage
Use:
Typical concentration to support conjugation in vitro is 5 - 20 μM (70-280 μg/ml) depending on conditions.
Storage:
Store at -80°C once reconstituted. Avoid multiple freeze/ thaw cycles.
Purity:
> 95% by SDS-PAGE