Biotin-E2-25K SUMO Conjugation Substrate
- 貨號 Cat. No.
- SP-205
- 規格 Size
- 25 μg
- 品牌 Brand
- Boston Biochem
Description
Biotin-E2-25K SUMO Conjugation Substrate
human recombinant
Product Information
25 μg
X mg/ml (X μM) in 50 mM HEPES pH 7.8, 50 mM NaCl, 10% glycerol, 1 mM DTT. Concentration will vary with specific lot number.
25 kDa
Use & Storage
Store at -80°C. Avoid multiple freeze/thaw cycles.
> 95% by SDS-PAGE
Catalog #: SP-205
Biotin-E2-25K SUMO Conjugation Substrate
Product Source:
human recombinant
This biotinylated protein can be used as a positive control for SUMOylation assays. It is modified by SUMO-1, SUMO-2 and SUMO-3 proteins and can be detected with avidin-linked reagents.
E2-25K is a class II ubiquitin-conjugating E2 enzyme identified as a target for SUMOylation during a screen for novel SUMO-1 targets. The SUMO modification of E2-25K has been extensively characterized in vitro (Pichler et al.). This substrate is efficiently SUMOylated by the SUMO activating E1 (SAE1/SAE2 or Aos1/Uba2) and the UbcH9 E2 enzyme in the absence of an E3 ligase. The E2-25K SUMO acceptor site is unique and does not conform to the ψKXE consensus site, being located at Lys14 and functioning only in the context of an α-helix.
Product Information
Quantity:
25 μg
Stock:
X mg/ml (X μM) in 50 mM HEPES pH 7.8, 50 mM NaCl, 10% glycerol, 1 mM DTT. Concentration will vary with specific lot number.
MW:
25 kDa
Use & Storage
Use:
Typical concentration to use in SUMO conjugation experiments in vitro is 1-5 μM depending on conditions.
Storage:
Store at -80°C. Avoid multiple freeze/thaw cycles.
Purity:
> 95% by SDS-PAGE