BioSim™ anti-Rituximab (Mabthera®) (Human) ELISA Kit

Alternate Name :

Tag Line :

A Sandwich ELISA kit for the quantitative measurement of antibody against Rituximab in human serum or plasma.

Storage Conditions: 4°C

Shelf life: 12 months

Summary :

• Detection method- Absorbance (450 nm)
• Species reactivity- Human
• Application- quantitative measurement of antibody against Rituximab in human serum and plasma

Detection Method: Absorbance (450 nm)

Features & Benefits:

Easy, convenient, sensitive and time-saving method to measure the level of antibody against Rituximab in human serum and plasma.
Detection Range: 62 - 500 ng/ml
Sensitivity: 30 ng/ml
Assay Precision: Intra-Assay: CV < 15%; Inter-Assay: CV < 15% (CV (%) = SD/mean X 100)
Cross Reactivity: Rituximab (Rituxan®, Mabthera®) infusion camouflages/masks the presence of antibody to rituximab (ATR) in serum/plasma samples. Therefore, blood sampling time is critical for detection of ATR. It is convenient to obtain blood sample just before the infusion or at least 2 weeks after the infusion of Rituximab (Rituxan®, Mabthera®)
Recovery rate: 85 – 115% with normal human serum samples with known concentrations

Description:

Rituximab (Mabthera®) is a genetically engineered chimeric murine/human monoclonal antibody specific to CD20. CD20 is an approximately 35 KDa transmembrane phosphoprotein involved in the activation, proliferation, and differentiation of B-lymphocytes. It is absent in terminally differentiated plasma cells. The Fab domain of rituximab binds to the CD20 antigen on B-lymphocytes and the Fc domain recruits immune effector functions to induce apoptosis in B cells. It is used in treating leukemias and lymphomas, some autoimmune disorders, and organ transplant. However, some patients develop unwanted immunogenicity, which leads to production of anti-drug-antibodies (ADAs) inactivating the therapeutic effects of the treatment and, in rare cases, inducing adverse effects. BioVision’s BioSimTM anti-Rituximab ELISA kit is designed to quantify/measure the antibody against Rituximab with high specificity and sensitivity in biological matrices.