BioSim™ anti-HER2 mab (Human) ELISA Kit

Alternate Name :

Tag Line :

A Sandwich ELISA kit for the quantitative measurement of antibody against Trastuzumab in human serum or plasma.

Storage Conditions: 4°C

Shelf life: 12 months

Summary :

• Detection method- Absorbance (450 nm)
• Species reactivity- Human
• Application- quantitative measurement of antibody against Trastuzumab in human serum and plasma

Detection Method: Absorbance (450 nm)

Features & Benefits:

Easy, convenient, sensitive and time-saving method to measure the level of antibody against Trastuzumab in human serum and plasma.
Detection Range: 31 - 500 ng/ml
Sensitivity: 30 ng/ml
Assay Precision: Intra-Assay: CV < 15%; Inter-Assay: CV < 15% (CV (%) = SD/mean X 100)
Cross Reactivity: Trastuzumab (Herclon®, Herceptin®) infusion camouflages/masks the presence of antibody to trastuzumab (ATT) in serum/plasma samples. Therefore, blood sampling time is critical for detection of ATT. It is convenient to obtain blood sample just before the infusion of Trastuzumab or at least 2 weeks after the infusion of Trastuzumab
Recovery rate: 85 – 115% with normal human serum samples with known concentrations

Description:

Trastuzumab (Herclon®, Herceptin®) is a recombinant DNA-derived humanized monoclonal antibody that selectively targets the extracellular domain of the human epidermal growth factor receptor 2 protein (HER2). Trastuzumab has antitumor activity against HER2-positive human breast tumor cells in laboratory models and is active for the treatment of women with HER2-overexpressing breast cancers. In HER2 overexpressing cells, trastuzumab markedly down-regulates HER2 expression by accelerating receptor endocytosis and degradation and inhibits cell cycle progression by inducing the formation of p27Kip1/Cdk2 complexes. However, some patients develop unwanted immunogenicity, which leads to production of anti-drug-antibodies (ADAs) inactivating the therapeutic effects of the treatment and, in rare cases, inducing adverse effects. BioVision’s BioSimTM anti-Trastuzumab ELISA kit is designed to quantify/measure the antibody against Trastuzumab with high specificity and sensitivity in biological matrices.