BioSim™ anti-Cetuximab II (Human) ELISA Kit

Alternate Name :

Tag Line :

A Sandwich ELISA kit for the qualitative measurement of antibodies to Cetuximab in human serum or plasma samples

Storage Conditions: 4ºC

Shelf life: 12 months

Summary :

Detection Method: Absorbance (450 nm)

Features & Benefits:

Cross Reactivity: Except for Cetuximab, there is no cross reaction with other therapeutic antibodies and native serum immunoglobins,Assay Precision: Intra-Assay and Inter-Assay CV < 30%,For in vitro qualitative determination of antibodies to Cetuximab in human serum and plasma samples

Description:

Cetuximab is a recombinant human/mouse chimeric monoclonal antibody that targets the epidermal growth factor receptor (EGFR) and acts as a competitive inhibitor for both epidermal growth factor and other ligands. Binding of the antibody to EGFR causes dimerization of the receptor and endocytosis which results in cell-cycle arrest and cell death. The monoclonal antibody is used to treat several forms of cancer such as metastatic colorectal cancer, non-small cell lung cancer, head and neck cancer. BioSim™ anti-Cetuximab ELISA kit has been specifically developed to qualitatively determine antibodies to Cetuximab in human serum and plasma samples. The ELISA kit is based on the sandwich principle. Controls and samples (serum and plasma) are incubated in the microtiter plate coated with the drug Cetuximab. After incubation, the wells are washed and the enzymatic activity is detected by the addition of chromogen-substrate. The enzyme-substrate reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of antibodies specific for Cetuximab present in the samples and controls. The qualitative test results can be evaluated using cut-off value.