BIF Antibody

Antibody Target :BIF-1

Host: Rabbit

Antibody Type: Polyclonal

Isotype: Rabbit IgG

Antibody Form: Affinity Purified Antibody

Immunogen Sequence: Synthetic peptide surrounding amino acid 351 of human BIF

Accession#: Q9Y371

Gene ID: 51100

Appearance: Colorless liquid

Concentration: 0.2 mg/ml

Formulation: 100 µg (0.2 mg/ml) Protein A affinity purified rabbit anti-BIF polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.

Purification: Affinity purified

Species Reactivity: hu/ms/rt

Application: WB

Application & Usage: Western blotting (2-6 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 40 kDa BIF-1 in samples from human, mouse, and rat origins. Jurkat cell lysate and rat kidney tissue lysates can be used as positive controls.

Storage Temp.: -20 °C

Shipping : gel pack

Shelf Life: 12 months

Handling: The antibody solution should be gently mixed before use.

Brief Background Descriptions: BIF-1 (Bax interacting factor-1) is a 365 amino acid protein that interacts with Bax protein and influences cell survival and death. BIF-1 contains a Src homology 3 (SH3) domain in the C-terminus. Overexpression of BIF-1 promotes Bax conformational change, caspase activation, and apoptotic cell death.