bFGF basic protein
- 貨號 Cat. No.
- bFGF basic protein
- 規格 Size
- 50ug
- 品牌 Brand
- gene-rl
Description
Product Name
bFGF basic protein
Product type
Proteins and Peptides
Size
50ug
Peptide
Recombinant full length protein, with a N-terminal hexahistidine
tag.
Tested applications
SDS-PAGE, Western Blotting, Cell Test
Application notes
Recommended dilutions
SDS-PAGE: Use at an assay dependent dilution
Western Blotting: Use at an assay dependent dilution
Optimal dilutions/concentrations should be determined by the end
user.
Relevance
FGF basic (FGF2) is a member of the fibroblast growth factor
(FGF) family. FGF family members bind heparin and possess
broad mitogenic and angiogenic activities. FGF2 is involved in
diverse biological processes, such as limb and nervous system
development, wound healing, and tumor growth. FGF2 mRNA
contains multiple polyadenylation sites, and is alternatively
translated from AUG and non-AUG (CUG) initiation codons
resulting in five different isoforms with distinct properties. The
CUG-initiated isoforms are localized in the nucleus and are
responsible for the intracrine effect, whereas, the AUG-initiated
form is mostly cytosolic and is responsible for the paracrine and
autocrine effects of this FGF.
Storage buffer
n/a
Purity
>95% by SDS-PAGE and PLC analysis.
Form
Lyophilised
Storage instructions
Aliquot and store at -20°C. Avoid freeze / thaw cycles.
Expression system
Yeast (Eukaryotic cell)
Sequence
Human FGF basic is a 17.2 kDa protein containing 155amino
acid residues:
MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF
LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN
RYLAMKEDGR LLASKCVTDE CFFF ERLESN NYNTYRSRKY
TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
0 ng/ml
200 ng/ml
2 ug/ml
2 ug/ml
Fig 1: bFGF Treatment of 3T3 Cell Line
　　Different concentration of purified bFGF was added into 3T3 cell culture medium.
　　Pictures were taken after 24 or 48 hours.
　　The purified growth factor increased the proliferation of 3T3 cell line.
ã€€ã€€ç”Ÿé•·å› å在3T3的細胞ä¸ç”¨ä¸åŒæ¿ƒåº¦åŸ¹é¤Š,æ–¼24或48å°æ™‚æ‹ç…§è§€å¯Ÿ
ã€€ã€€ç”Ÿé•·å› åå¯å¢žåŠ 3T3分化生長的能力
Fig2: Affinity Purification bFGF
　　protein was purified by Fast
　　Flow affinity column.
　　bFGF蛋白質經由Fast Flow親
　　åˆæ€§ç®¡æŸ±ç´”化
Fig3:(a) Western Blotting of bFGF
　　The blotting membrane was hybridized
　　with anti-bFGF antibody to check the purity.
　　利用bFGF的抗體來檢查轉漬膜
(b)SDS Page of bFGF
　SDS page was used to check the bFGF
　molecular weight and purity.
　使用SDS Page來檢測bFGF的分åé‡
　大å°åŠç´”度