Betaxolol HCl

Description

Name: Betaxolol HCl
Brand: Selleckchem
SKU: S1827

Description

Betaxolol is a β1 adrenergic receptor blocker with IC50 of 6 μM.

β1 adrenergic receptor

In vitro

Betaxolol is able to protect retinal neurones. [2] Betaxolol attenuates the NMDA-induced influx of 45Ca2+ while β-adrenoreceptor agonists are ineffective. [3] The glutamate-induced release of LDH is almost completely prevented when betaxolol (10 μM) is included. [4] Betaxolol (100 μM) is very effective in preventing the hypoxia-induced release of LDH from cortical cultures. [5]

When Betaxolol is injected i.p. into the rats before ischaemia and on the days of reperfusion the changes to the calretinin and ChAT immunoreactivities are reduced and the reduction of the b-wave is prevented. Inclusion of betaxolol partially prevents the changes caused by NMDA and lack of oxygen/glucose. [5]

Betaxolol HCl

Catalog No.S1827

Betaxolol is a β1 adrenergic receptor blocker with IC50 of 6 μM.

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Cell lines	Dissociated rat cortical cells
Concentrations	100 μM
Incubation Time	4 hours
Method	Dissociated cortical cells from 16–18-day-old fetal rats are grown, in 35 mm dishes, in DMEM supplemented with L-glutamine (4 mM), glucose (6 g/L), penicillin (100 U/mL), streptomycin (100 μg/mL) and 10% hormonal supplemented medium consisting of transferrin (1 mg/mL), insulin (250 μg/mL) putrescine (600 μM), sodium selenite (0.3 μM), progesterone (0.2 μM) and estradiol (0.1 pM) for 7 days in an atmosphere of 5% CO2/95% O2at 37 °C. The cultures are then transferred to a culture medium which lacks the hormonal supplemented medium. L-glutamate is added to the medium and incubated for a further 4 hours under normoxic conditions. Betaxolol are added to the cultures at the same time as L-glutamate. In other experiments the cultures are subjected to anoxic conditions, 95% N2/5% CO2, for 5 hours at 37 °C. Betaxolol is added prior to anoxia. Reoxygenation is then achieved by replacing the cells in normoxic conditions (95% O2/5% CO2) for 3 hours. Cellular injury is assessed by measuring lactate dehydrogenase (LDH) release into the cell culture supernatant after hypoxia/reoxygenation or glutamate exposure. LDH activity is assayed spectrophotometrically by following NADH metabolism for 2 minutes at 340 nm.

Solubility (25°C) *	In vitro	DMSO	69 mg/mL (200 mM)
		Water	69 mg/mL (200 mM)
		Ethanol	69 mg/mL (200 mM)
	In vivo	Saline,	30 mg/mL
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.