Beta-Secretase Fluorometric Assay Kit

100 assays. The beta-Secretase activity Assay Kit provides a convenient fluorescence method for detecting beta-secretase activity in biological and purified samples. The assay utilizes a secretase-specific peptide conjugated to two reporter molecules EDANS and DABCYL. In the uncleaved form, the fluorescent emissions from EDANS are quenched by the physical proximity of the DABCYL moiety. Cleavage of the peptide by secretase physically separates EDANS and DABCYL allowing for the release of a fluorescent signal.