Beta-Secretase Activity Fluorometric Assay Kit
Kits
- 貨號 Cat. No.
- K360-100
- 規格 Size
- K360-100
- Sample Type
- as well as many other biological fluids 、 Cell and tissue lysates 、 culture media 、 Plasma and Serum 、 urine
Description
Alternate Name : BACE1 Activity Fluorometric Assay Kit, ASP2 Activity Fluorometric Assay Kit, Memapsin-2 Activity Fluorometric Assay Kit
Tag Line :
Highly Sensitive Assay
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Detection method- Fluorescence (Ex/Em 335-355/ 495-510 nm)
• Species reactivity- Mammalian
• Applications- Convenient fluorescence method for detecting β-secretase activity in biological and purified samples.
Detection Method:
Features & Benefits:
• Simple procedure; takes ~ 1 hour
• Fast and convenient
• The assay is sensitive, stable and high-throughput adaptable.
Description:
β-Secretase has been implicated to be an excellent target for anti-amyloid therapy for the treatment of Alzheimer’s disease. The β-Secretase activity Assay Kit provides a convenient fluorescence method for detecting β-secretase activity in biological and purified samples. The assay utilizes a secretase-specific peptide conjugated to two reporter molecules EDANS and DABCYL. In the uncleaved form, the fluorescent emissions from EDANS are quenched by the physical proximity of the DABCYL moiety. Cleavage of the peptide by secretase physically separates EDANS and DABCYL allowing for the release of a fluorescent signal. The level of secretase enzymatic activity in samples is proportional to the level of fluorescence intensity.