Beta-Lactamase Activity Colorimetric Assay Kit
Kits
- 貨號 Cat. No.
- K803-100
- 規格 Size
- K803-100
- Sample Type
- • Serum 、 bacterial cultures 、 etc.) 、 saliva from mammals infected with βL-secreting bacteria • Food (e.g. milk) • Fermentation media 、 urine
Description
Alternate Name :
Tag Line :
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Detection method: Absorbance (490 nm)
• Application:
- Measurement of β-Lactamase activity in various biological samples
- Analysis of β-Lactamase activity in pathological conditions
Detection Method: Absorbance (490 nm)
Features & Benefits:
• Rapid, simple & sensitive
• The assay can detect enzymatic activity as low as 0.06 mU in a variety of biological samples.
Description:
Beta-Lactamases (βLs), are a large family of hydrolases comprising more than 850 identified members expressed in Gram-positive and Gram-negative bacteria. βLs can be classified according to their substrate or inhibitor specificity. These enzymes are capable of hydrolyzing four atom rings known as β-lactams. Antibiotics containing β-lactam rings (i.e. penicillin, cephalosporin, monobactam, carbapenem) are highly susceptible to be hydrolyzed via enzymatic activity, which deactivates their antibiotic potency. βLs have become a significant clinical threat due to the alarming number of cases of bacterial strains showing β-lactam antibiotic resistance. BioVision’s Beta-Lactamase Activity Assay Kit offers a simple and sensitive assay that can detect and quantify the enzymatic activity of these hydrolases. The assay is based on the hydrolysis of Nitrocefin, a chromogenic cephalosporin, that results in the generation of a colored product (OD 490 nm), which is directly proportional to the amount of βL activity. The assay can detect enzymatic activity as low as 0.06 mU in a variety of biological samples.