Beta Galactosidase (β-Gal) Inhibitor Screening Kit (Fluorometric)

Alternate Name : Elastin receptor 1 assay kit

Tag Line :

Market's first and only kit for screening beta galactosidase Inhibitors. Simple, HTP compatible, 30 minutes protocol.

Storage Conditions: -20°C

Shelf life: 12 months

Summary :

A simple and high-throughput adaptable assay kit used to fluorometrically (Ex/Em = 480/520 nm) screen/study/characterize potential inhibitors of beta galactosidase within 30 min.

Detection Method: Fluorescence (Ex/Em = 480/520 nm)

Features & Benefits:

Simple and High throughput adaptable
30 minutes short protocol
Detect using a fluorometer or fluorescence microplate reader.

Description:

Beta Galactosidase (β-Gal, EC: 3.2.1.23) is an enzyme which hydrolyzes the β-galactosides into monosacchanides. β-Gal is widely used as a reporter gene in the field of molecular biology. Senescence Associated β-Gal (SA-β-Gal) is an isoform of β-Gal which has the optimal activity at pH 6.0, and is mostly used as a biomarker for senescent cells (K802). β-Gal is an essential enzyme in humans and its deficiency results in Morquio’s Syndrome, a severe birth defect. β-Gal can also be used as a tool to study protein-protein interaction. In BioVision’s Beta-Galactocidase Inhibitor Screening Kit, β-Gal converts β-Gal substrate to give an intensely fluorescent product (Ex/Em = 480/520 nm). In the presence of a β-Gal inhibitor, the reaction is impeded/abolished resulting in decrease or total loss of fluorescence. This assay kit can be used to screen/study/characterize the potential inhibitors of Beta Galactosidase. The assay is simple, high-throughput adaptable and can be performed within 30 min.