BDS-II
- 貨號 Cat. No.
- B-450
- 規格 Size
- 5 μg 5x5 μg 10μg 5x10μg 0.1mg
Description
BDS-II
A Specific Blocker of Kv3 Channels
| Product#: | B-450 |
| Sizes: |
| 5 mg |
| 5 x 5 mg |
| 10 mg |
| 5 x 10 mg |
| 0.1 mg |
BDS-II is a 43 amino acid peptidyl toxin isolated from the sea anemone Anemonia sulcata venom. BDS-II was shown to be a specific Kv3.4 blocker. BDS-II blocked 70% of the Kv3.4 current in COS-transfected cells at a concentration of 2.8 mM. The blocking effect is rapid, direct and reversible.1 .Recently it was shown that it blocks other Kv3 channels with similar potencies.2 BDS II inhibited the A type K+ current in human pulmunary artery smooth muscle cells.3 In skeletal muscle cell line (C2C12), BDS II inhibited single KV channel currents and shifted the resting membrane potential in a dose dependent manner and in a similar way it inhibited KV3.4 channels co-expressed with the KCNE3 auxiliary subunit in CHO cells. The inhibition of these channels was about ten times less potent than the inhibition of KV3.4 channels expressed alone (IC50 of about 1 mM and 10 mM respectively).4
| M.W.: | 4783 |
| Sequence: | AAPCFCPGKP DRGDLWILRG TCPGGYGYTS NCYKWPNICC YPH |
| Purity: | >95% |
| Bioassay: |
| The biological activity of BDS-II was tested on Kv3.4 K+ channel expressed in Xenopus oocytes. |
 |
Inhibition of Kv3.4 channels expressed in Xenopus oocytes. Left: example traces before (red) and during (black) bath perfusion of 1 mM BDS-II (Cat. # B-450). Holding potential was ?00 mV, test potential to 0 mV (100 ms) was delivered every 10 seconds. Recordings were made while perfusing ND96 Buffer. Right: time course for the experiment shown on the left. The vertical bar indicates the period of BDS-II perfusion. |
| References: |
1. | Diochot, S. et al. (1998) J. Biol. Chem. 273, 6744. |
2. | Yeung, S. Y. M. et al. (2005) J. Neurosci. 25, 8735. |
3. | Iida, H. et al. (2005) British. J. Pharmacol. 146, 49. |
4. | Abbott, J. W. et al. (2001) Cell 104, 217. |