BDS-I
- 貨號 Cat. No.
- B-400
- 規格 Size
- 5 μg 5x5 μg 10μg 5x10μg 0.1mg
Description
BDS-I
A Specific Blocker of Kv3 Channels
| Product#: | B-400 |
| Sizes: |
| 5 mg |
| 5 x 5 mg |
| 10 mg |
| 5 x 10 mg |
| 0.1 mg |
Anemonia sulcata venom. It is reported to be a selective blocker of Kv3.4 K+ channel. BDS-I blocked 60% of the Kv3.4 current in COS-transfected cells at a concentration of 2.5 mM. The blocking effect is rapid, direct and reversible.1 Recently it was shown that it blocks other Kv3 channels with similar potencies.2 BDS-I inhibited KV currents in carotid body cells3 an effect, which disapeared after chronic hypoxia. Shuch a result helped establishing the unique role played by KV3 channels in the response to hypoxia.4 BDS I (2.5 mM) also reduced the native transient K+ current and increased the action potential duration in hippocampal granule neurons.5 In corneal epithelial cells BDS I (400 nM) inhibited most of the detected KV current.6 In magnocellular neurosecretory neurons of the hypothalamus 100 nM BDS I, inhibited about half of the KV current and increased the action potential duration.7 In fast spiking neurons from different brain areas, 2 mM BDS I inhibited part of the KV current and broadened the action potential and reduced spike freequency.8
| M.W.: | 4715 |
| Sequence: | AAPCFCSGKP GRGDLWILRG TCPGGYGYTS NCYKWPNICC YPH |
| Purity: | >95% |
| Bioassay: |
| The biological activity of BDS-I was tested on Kv3.4 K+ channel expressed in Xenopus oocytes. |
 |
Inhibition of Kv3.4 channels expressed in Xenopus oocytes. Left: example traces before (red) and during (black) bath perfusion of 1 mM BDS-I (Cat. # B-400). Holding potential was ?00 mV, test potential to 0 mV (100 ms) was delivered every 10 seconds. Recordings were made while perfusing ND96 Buffer. Right: time course for the experiment shown on the left. The vertical bar indicates the period of BDS-I perfusion. |
| References: |
1. | Diochot, S. et al. (1998) J. Biol. Chem. 273, 6744. |
2. | Yeung, S. Y. M. et al. (2005) J. Neurosci. 25, 8735. |
3. | Sanchez, D. et al. (2002) J. Physiol. 542, 369. |
4. | Kaab, S. et al. (2005) J. Physiol. 566, 395. |
5. | Riazanski, V. et al. (2001) J. Physiol. 537, 391. |
6. | Wang, L. et al. (2004) Invest. Ophthamol. Vis. Sci. 45, 1796. |
7. | Shevchenko, T. et al. (2004) J. Neurophysiol. 92, 3043. |
8. | Baranauskas, G. et al. (2004) Nature Neurosci. 6, 258 |
BDS-I is a 43 amino acid peptidyl toxin isolated from the sea anemone