BCL2 Antibody (LS-B2352)

貨號 Cat. No.: LS-B2352
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2352
Antibody Validation:	This antibody replaces catalog number LS-C20871. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Apoptosis Regulator Bcl-2 (BCL2)
Synonyms:	BCL2, B-cell CLL/lymphoma 2, B-cell lymphoma protein 2, bcl-2, bcl2
Family:	Apoptosis
Subfamily:	Not assigned
Host:	BCL2 antibody was produced in Mouse
Antigen Species:	BCL2 antibody was raised against Human
Clonality:	Monoclonal
Isotype:	IgG1
Antigen Type:	Synthetic peptide
Immunogen:	BCL2 antibody was raised against synthetic peptide corresponding to residues 41-54 (Cys-GAAPAPGIFSSQPG) of human bcl-2 protein. Cellular Localization: Cytoplasm. MW of antigen: 25-26kD
Antibody Specificity:	Recognizes human Bcl-2a at ~25kD. Species cross-reactivity: monkey. Does not react with mouse or rat.
Antigen Modification:	aa41-54
Predicted Species Reactivity:	Monkey, Porcine
Purification:	Protein G Column
Presentation:	PBS, pH 7.4
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2352 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2352 was determined to be 5 ug/ml. Western Blot: 1 ug/ml for 2hrs at RT. Positive control: Tonsil or follicular lymphomas. Raji or HeLa cells.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (1 µg/ml), Flow Cytometry (Flo), Immunofluorescence (IF), ELISA (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	1 mg/ml