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Basic Antigen Affinity-Purified Polyclonal Antibody Package 基本型抗原親和純化多克隆抗體代製(兔子)

貨號 Cat. No.
SC1247
規格 Size
客製化

Description

不同於單克隆抗體，多克隆抗體對應於多表位，往往包含若干免疫球蛋白亞型。它們可以用於Westerns,ELISAs and IHC。我們保證由GenScrip做肽抗原設計，合成及免疫的抗體在任何物種於ELISA將有稀釋比例1:20,000或更好。對於任何蛋白抗原（3毫 克， “純度80％，90％首選），我們保證了積極的抗體免疫印跡結果，並用ELISA稀釋比例1:20,000或更好。否則我們將免費重新啟動該項目免費， 直至保證滿意。我們的動物設施經過AAALAC認證的國際認可。


























Service Package
Peptide Antigen
Protein Antigen
Includes

  • Conjugation to KLH

  • 2 rabbits, pre-immune bleed, 4 immunizations/rabbit

  • Final bleed

  • Peptide conjugated to affinity resin

  • Affinity purification of antibodies

  • ELISA test

  • Lyophilization

  • Antigen provided by the customer: 7.5-8 mg of peptide (2.5-3 mg for immunization, 5 mg for purification)

  • Protein checking by SDS-PAGE

  • 2 rabbits, pre-immune bleed, 4 immunizations/rabbit

  • Final bleed

  • Protein conjugated to affinity resin (Dialyze the protein with PBS if needed)

  • Affinity purification of antibodies

  • ELISA test

  • Lyophilization

  • Antigen provided by the customer: 7.5-8 mg of protein (2.5-3 mg for immunization, 5 mg for purification).The concentration should be >0.4 mg/ml, and no organic solvents in the antigen solution. Tris and other solution containing amino groups should not be used.
Delivery

  • 1-2 ml of pre-immune serum (lyophilized)*

  • ELISA results

  • 3-5 mg affinity-purified antibodies (lyophilized)

  • Remaining antigen

  • 1-2 ml of pre-immune serum (lyophilized)*

  • ELISA results

  • 3-5 mg affinity-purified antibodies (lyophilized)

  • Remaining antigen
Time Frame

  • Conjugation: 1 week

  • Immunization: 10 weeks

  • Purification and ELISA: 1 week

  • Lyophilization and Shipping: 1-2 weeks

  • Total 13-14 weeks

  • Protein checking: 1 week

  • Immunization: 10 weeks

  • Purification and ELISA: 1 week

  • Lyophilization and Shipping: 1-2 weeks

  • Total 13-14 weeks

備註


如果沒有具體要求，0.02% w/v sodium azide將被添加在抗血清或抗體產品，以防止細菌滋生。凍乾延長了抗體的壽命，消除了漏水問題，減輕了嚴格的運輸溫度要求，減少了抗體的總重量。提供所有的抗血清或純化抗體凍乾形式，除非特殊的要求.

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