B27M2
- 貨號 Cat. No.
- CLC004
- 品牌 Brand
- gene-rl
Description
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Cell Lines (GRL-CLC004) | |
Designations | B27M2 |
| Cell type | Hybridoma |
Growth property | Suspension |
Seeding density(cells/ml) | 2X106 |
Survival rate | 95% |
Culture medium | 90% Modified RPMI 1640 medium with 50uM 2-mercaptoethanol+10% fetal bovine serumRPMI 1640 100ml L-Glutamine(100x) 1ml Solution FM 1ml Fetal Bovine Serum 12ml Non-essential amino acids(100x) 1ml |
| Incubation condition | 370C 5% CO2 |
Freezed medium | 95% culture medium+5% DMSO |
Medium renewal | every 2 to 3 days |
| Subculturing | Cultures can be maintained by addition or replacement of fresh medium. |
Mycoplasma test | Negative for bacteria，fungi and mycoplasma. |
Cellular products | Anti HLA-B27 and HLA-BW47 |
Comments | Cultures can be established by centrifugation with subsequent resuspension at 5-6x104 viable cells/ml Maintain cell density between 5x104 and 5x105 viable cells/ml |
Advertence: |
When you receive the cell line, please examine the flask and its cap. If it is not well-found, please tell us instantly. After examination, please wipe the flask and flask cap with 70% alcohol, and then loosen it. Afterward, put the flask into incubator immediately.