Proteins and Peptides
ATG7 Antibody (LS-B2215)
- 貨號 Cat. No.
- LS-B2215
- 規格 Size
- 50 µg
- 品牌 Brand
- LSBio
Description
| Antibody Catalog ID: | LS-B2215 |
|---|---|
| Antibody Validation: | This antibody replaces catalog number LS-C34998. It has been validated for use in the following assays: IHC-P. |
| Antibody Target: | Ubiquitin Activating Enzyme E1-like Protein (GSA7) (ATG7) |
| Synonyms: | ATG7, GSA7, ubiquitin activating enzyme E1-like protein |
| Host: | ATG7 antibody was produced in Rabbit |
| Antigen Species: | ATG7 antibody was raised against Human |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Antigen Type: | Synthetic peptide |
| Immunogen: | ATG7 antibody was raised against synthetic peptide corresponding to 17 amino acids near the carboxy terminus of human APG7 (Genbank accession No. NP_006386). |
| Antibody Specificity: | Reacts with human and mouse APG7 |
| Antigen Modification: | C-Terminus |
| Predicted Species Reactivity: | Mouse |
| Purification: | Immunoaffinity Chromatography |
| Presentation: | PBS, 0.02% sodium azide. |
| Recommended Storage: | Short term: 4º C, Long term: add sterile glycerol (40-50%), aliquot to avoid repeated freezing and thawing, and store at -20º C. |
| Antibody Usage Summary: | Immunohistochemistry: LS-B2215 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2215 was determined to be 5 ug/ml. Positive control: Caco-2 cell lysate. |
| Antibody Uses: | Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (0.5 - 1 µg/ml) (Optimal dilution to be determined by the researcher) |
| Size: | 50 µg |
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