Ataxin UIM agarose
- 貨號 Cat. No.
- AM-115
- 規格 Size
- 0.25 ml
- 品牌 Brand
- Boston Biochem
Description
Ataxin UIM agarose
human recombinant
Product Information
0.25 ml
UIM covalently coupled to agarose at 4 mg/ml resin (500 μM). Resin supplied in 50 mM Hepes pH 7.5, 250 mM NaCl.
Use & Storage
Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.
Catalog #: AM-115
Ataxin UIM agarose
Product Source:
human recombinant
Ataxin-3 protein belongs to a novel group of cysteine proteases similar to USP-type ubiquitin proteases and has deubiquitinating activity in vitro. The full-length protein contains an catalytic N-terminal Josephin domain, three ubiquitin interacting motifs (UIMs), and a variable C-terminus with a polyglutamine stretch. Ataxin-3 has deconjugating activity and functions as a mixed linkage, chain editing enzyme with preferential cleavage of K63 linkages in mixed chains. Ataxin3 also bind both K48-linked and K63-linked poly-Ub chains via its UIM domains and preferentially interacts with four or more ubiquitin units. This affinity resin can be used for the enrichment, isolation and identification of K48-linked (preferentially) or K63-linked poly-Ub chains or ubiquitinated substrates that contain these linkages.
Product Information
Quantity:
0.25 ml
Stock:
UIM covalently coupled to agarose at 4 mg/ml resin (500 μM). Resin supplied in 50 mM Hepes pH 7.5, 250 mM NaCl.
Use & Storage
Use:
Equilibrate resin by washing with 5-10 ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.
Storage:
The agarose can be re-used for at least 2 applications if properly maintained. After use, clean resin with 5 ml 50 mM Tris pH 9.0, 1 M KCl. Remove cleaning solution by washing resin with 5 ml storage buffer. Resin should be stored at 4°C, 0.01% sodium azide can be added as a bacteriostatic agent. DO NOT FREEZE.