Aspartate Aminotransferase (AST) (Mouse) ELISA Kit

Alternate Name :

Tag Line :

A Sandwich ELISA kit for the quantitative measurement of human AST in serum, plasma, culture supernatants and other biological fluids.

Storage Conditions: 4°C

Shelf life: 6 months

Summary :

• Detection method- Absorbance (450 nm)
• Species reactivity- Mouse
• Application- quantitative measurement of total AST in Mouse serum, plasma, tissue homogenates and other biological fluids.

Detection Method: Absorbance (450 nm)

Features & Benefits:

Easy, convenient and time-saving method to measure the level of AST in Mouse serum, plasma, tissue homogenates, culture supernatants and other biological fluids.
Detection Range: 0.313 - 20 ng/ml
Sensitivity: < 0.188 ng/ml
Assay Precision: Intra-Assay: CV < 8%; Inter-Assay: CV < 10%

Description:

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. BioVision’s AST ELISA kit is a sandwich ELISA assay for the quantitative measurement of AST in Mouse serum, plasma and cell culture supernatants. The density of color is proportional to the amount of Mouse AST captured from the samples.