AQP2 Antibody (LS-B2152)

貨號 Cat. No.: LS-B2152
規格 Size: 50 Âµl
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2152
Antibody Validation:	This antibody replaces catalog number LS-C49093. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Aquaporin 2 (Collecting Duct) (AQP2)
Synonyms:	AQP2, Aqp2, Aquaporin 2
Family:	Transporter
Subfamily:	Aquaporin
Host:	AQP2 antibody was produced in Rabbit
Antigen Species:	AQP2 antibody was raised against Rat
Clonality:	Polyclonal
Antigen Type:	Synthetic peptide
Immunogen:	AQP2 antibody was raised against phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser261 of rat aquaporin 2
Antibody Specificity:	Specific for ~29k AQP2 protein phosphorylated at Ser261. Also recognizes the glycosylated form of AQP2 at ~37k. Immunolabeling of the AQP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephosphopeptide.
Antigen Modification:	pSer261
Purification:	Immunoaffinity Chromatography
Presentation:	0.099 ml in 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg per ml BSA, 50% glycerol.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2152 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2152 was determined to be 1:100.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (1:100), Western blot (WB) (1:1000) (Optimal dilution to be determined by the researcher)
Size:	50 µl