Apoptotic Cell Isolation Kit

30 isolations. The kit provides a simple and efficient means for isolation of apoptotic cells from normal culture using annexin V magnetic beads (MagBeads). Annexin V is a Ca2+-dependent phospholipid binding protein with high affinity for phosphatidylserine (PS). During apoptosis PS is redistributed from the inner to the outer surface of plasma membrane. Once on the cell surface PS becomes available for binding to annexin V. Binding of annexin V-biotin to apoptotic cells followed by binding of the biotin to streptavidin-MagBeads enables separation of apoptotic cells from normal cells. The apoptotic cells bound to the MagBeads adhere to the magnet-while normal cells stay in suspension. The bound apoptotic cells can then be released from the MagBeads using the Elution Buffer provided in the kit. The separated apoptotic cells and normal cells can be used in a variety of assays to study apoptotic mechanisms and pathways. The kit has also been successfully used to remove dead cells from normal culture.