ApoDIRECT DNA Fragmentation Assay Kit
Kits
- 貨號 Cat. No.
- K402-50
- 規格 Size
- K402-50
- Sample Type
- Cell cultures (adherent and suspension)
Description
Alternate Name :
Tag Line :
Detects DNA Fragmentation by FACS or FL
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Detection method- Flow cytometry (Ex/Em = 488/520 nm for FITC, and 488/623 nm for PI) and fluorescence microscopy (FITC and rhodamine filters)
• Species reactivity- Mammalian
• Applications- The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.
Detection Method:
Features & Benefits:
• Simple procedure; takes ~ 1 hour
• Fast and convenient
• The assay is sensitive and stable
• The assay offers an one-step labeling of apoptotic cells.
Description:
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s ApoDIRECT In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The TUNEL-based detection kit utilizes terminal deoxynucleotidyl transferase (TdT) to catalyze incorporation of fluorescein-12-dUTP at the free 3’-hydroxyl ends of the fragmented DNA. The fluorescein-labeled DNA can then be observed by fluorescence microscopy or analyzed by flow cytometry.