ApoBrdU-IHC DNA Fragmentation Assay Kit
Kits
- 貨號 Cat. No.
- K403-50
- 規格 Size
- K403-50
- Sample Type
- Tissue sections and fixed cell preparations
Description
Alternate Name :
Tag Line :
Two-Color TUNEL Assay
Storage Conditions: -20°C
Shelf life: 12 months
Summary :
• Species reactivity- Mammalian
• Applications- The TUNEL-based immunohistochemical staining kit provides complete reagents including positive and negative control slides for convenient detection of DNA fragmentation
Detection Method:
Features & Benefits:
• Simple procedure; convenient
• The assay is sensitive and stable
• The assay offers an one-step labeling of apoptotic cells.
Description:
Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells. BioVision’s Apo-BrdU-IHCTM Kit is a two-color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labeling) assay for labeling DNA breaks to detect apoptotic cells by immunohistochemistry. The kit contains positive/negative control slides for assessing reagent performance; reaction and blocking buffers for processing individual steps in the assay; proteinase K; terminal deoxynucleotidyl tranferase enzyme (TdT), bromodeoxyuridine triphosphate (Br-dUTP), biotin labeled antiBrdU antibody for labeling DNA breaks, horseradish peroxidase streptavidin conjugate, DAB, H₂O₂/Urea tablets for color generation and methyl green solution for counter staining the cells.