APOB Antibody (LS-B2616)

貨號 Cat. No.: LS-B2616
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2616
Antibody Validation:	This antibody replaces catalog number LS-C20721. It has been validated for use in the following assays: IHC-P.
Antibody Target:	Apolipoprotein B-100 (APOB)
Synonyms:	APOB, apo b-100, apo b-48, ApoB-100, ApoB-48, Apolipoprotein B, apolipoprotein b-100, apolipoprotein b-48, FLDB, LDLCQ4
Host:	APOB antibody was produced in Goat
Antigen Species:	APOB antibody was raised against Human
Clonality:	Polyclonal
Isotype:	IgG
Antigen Type:	Purified protein
Immunogen:	APOB antibody was raised against human Apolipoprotein Type B (isolated from human plasma by density gradient centrifugation followed by HPLC purification)
Antibody Specificity:	Recognizes human apolipoprotein B. Assay by IEP resulted in a single precipitin arc against anti goat serum. Has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apolipoproteins. Typically less than 1% cross-reactivity against other types of apolipoprotein was detected by ELISA against purified standards. Non-specific cross-reaction of anti-apolipoprotein antibodies with other human serum proteins is negligible.
Purification:	Immunoaffinity Chromatography
Presentation:	0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA, pH 8, 0.01% sodium azide.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2616 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D3, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2616 was determined to be 5 ug/ml.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml), Western blot (WB) (1:1000 - 1:5000), Immunoprecipitation (IP), Gel shift (GS), ELISA (1:1000 - 1:5000) (Optimal dilution to be determined by the researcher)
Size:	50 µg
Concentration:	1 mg/ml